Pillbug Reaction In the Presence of an Acid Abstract: This experiment was conducted to learn how regular behavior of a pillbug is affected in the presence of a substance such as hydrochloric acid. Behavior of the pill bugs was first monitored and recorded to discern how the species acts under normal conditions. The behavior was then observed and documented after the pill bugs were placed in a choice chamber with HCl and water. There was no change in activity after these substances were placed with the pill bugs.
From this experiment, we can conclude not only that pill bug behavior goes unchanged in the presence of water, but also that the behavior stays consistent in the presence of hydrochloric acid. However, the HCl in this experiment was diluted to a pH of 2, and we have yet to test differing dependent factors on these pill bugs. Introduction: Ethology, the study of animal behavior, requires several factors for completing an experiment successfully. These factors may include research of a species, observation of an organism, interpretation of observation, and analysis of said species reaction to stimuli or unfamiliar conditions.
Two commonly studied animal behaviors are taxis, a deliberate movement toward or away from stimuli, and kinesis, a random movement that is not directed toward or away from stimuli. Based on this previous knowledge, we are hypothesizing that the pill bugs in our study will undergo taxis and deliberately move away from hydrochloric acid. Materials and Methods: To perform this experiment, ten pill bugs were placed in a choice chamber labeled side A and B with masking tape, and observed for at least ten minutes.
Filter paper with water was placed on side B, and filter paper with hydrochloric acid was placed on side A. Behavior was monitored and recorded for the next ten minutes, and conclusions were met. Results: The dependent variables for this experiment included the number of pill bugs present strength of the acid (pH of 2). The independent variable was the amount of HCl, and the controls included the number of pill bugs present (ten) and the amount of time results were recorded (ten minutes). One confounding variable for this experiment was the different species of pill bugs.
Different species may or may not have been attracted to different variables, being a possible source for error. Results and observations were recorded as follows: Data Analysis: Table 1 list general observations when the pill bugs were initially observed, and a pill bug drawing is shown in Table 2. Table 3 lists the number of pill bugs in each side of the choice chamber during a time span of ten minutes; numbers are recorded every one minute. Table 4 records the number of pill bugs in each side under altered environmental conditions.
Graph 1 shows differences in numbers of pill bugs between sides A and B. The Chi Square Goodness of Fit Test analyzes the preference, or lack thereof, for pill bugs to regular environmental conditions or those altered with an acid. Results for this test show that the pill bugs had no preference for the hydrochloric acid. Table 1: General Observations Table 2: Drawing of Pillbug Graph 1: Difference in Number of Pill bugs on Side A and B Key Table 3: Pill Bug Taxis: Time (min)Number of Pill bugs in side ANumber of Pill bugs in side B 055 64 282 3100 455 537 655 773 846 946 1064 Average5. 74. 3 Class Average Data Analysis 1: Chi Square Goodness of Fit Test Control: Experimental: Table 4: Pill Bug Taxis with Altered Environmental Conditions: Condition TestedWaterHCl Test (min) Number of Pill bugs in side ANumber of Pill bugs in side B 055 146 255 346 464 555 637 746 846 946 1055 Conclusion: From this experiment, we have observed that the behavior of the pill bug was unaffected in these altered environmental conditions; altered conditions being the HCl added to side A of the choice chamber.
The pill bugs stayed on each side consistently, regardless of the hydrochloric acid and water covered filter paper. We can conclude that while pill bugs may undergo taxis in many conditions, the pill bugs attraction to water and HCl was low. Therefore, any behavioral changes in their altered environmental conditions can be considered kinesis because they had no direct route towards this stimuli. Based on data and statistical methods, our evidence does not support out hypothesis that the pill bugs will undergo taxis and deliberately move away from hydrochloric acid.